Demo

A complete PlasValidate report for a real Oxford Nanopore plasmid run, with no account needed. It is the same report the pipeline produces for every sample: plasmid map, annotated features, the QC checklist with each check shown against its threshold, and the per-base read-support viewer. The example is barcode01 of Oxford Nanopore's public plasmid_2025.04 dataset.

The report is a single self-contained file: download it and it opens offline in any browser.

Run it yourself, no account. Assemble a real Oxford Nanopore plasmid through the live pipeline in one click. The run is queued at the lowest priority, so signed-in users' runs go first; a live progress page opens straight away and becomes the finished report, at its own shareable link, once the run completes.

The FASTQ above is exactly the input this run assembles, so you can inspect the raw data yourself. When the report is ready, choose Compare to a design and upload the example design to check the sequence against it. To validate your own reads, use the upload below.

Validate your own reads, no account. Upload a folder of Oxford Nanopore FASTQ files (the fastq_pass folder, or a single barcode) and PlasValidate assembles them through the live pipeline at the lowest priority, with no reference or expected size needed. You land on your run's own shareable, login-free page, which lists every sample and opens each report as it finishes. Without an account a run can hold up to 96 barcodes and 5 GB of reads, and one run at a time can be in progress from each internet address. For larger sets, create a free account.

Choosing files instead of a folder: files whose names carry a barcode (as MinKNOW names them) go to that barcode; the rest are one sample.

What this example shows. The plasmid was assembled de novo from the reads alone, with no reference sequence and no expected size supplied. The verdict, the size, and the feature annotation are all derived from the read evidence.

This report was computed in advance, so it opens straight away. To run the pipeline yourself, use Run sample data or upload your own reads above; no account is needed for either.

PlasValidate also runs entirely from the command line, with no portal and no account, via the validation-engine command. Installation and usage are in the Command line section of the documentation.